ATP-mediated intrinsic peroxidase-like activity of Fe3O4-based nanozyme: One step detection of blood glucose at physiological pH

May 01, 2017

Fe3O4 nanoparticles (Fe3O4 NPs), demonstrating peroxidase-like activity has garnered attention in the detection of several biomolecules, therefore, emerged as an excellent nano-biosensing agent. The intrinsic peroxidase-like activity of Fe3O4 NPs at acidic pH is the fundamental action driving the oxidation of substrates like TMB, resulting in a colorimetric product formation used in the detection of biomolecules. Hence, the detection sensitivity essentially depends on the ability of oxidation by Fe3O4 NPs in presence of H2O2. we extended single step detection method to monitor glucose level in human blood serum and detected in a time span of <5 min at pH 7.4.

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Keywords

Iron-oxide nanoparticles, Nanozymes, Point-of-care device, Horseradish peroxidase, Ascorbic acid
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