Dr Neha Jain is an Assistant Professor in the Biological and Life Sciences division of the School of Arts and Sciences. She did an MSc in Industrial Microbiology at the University of Rajasthan, Jaipur, and a PhD in Molecular Biophysics at the Indian Institute of Science Education and Research, Mohali (IISER Mohali). Her PhD work was focused on understanding the mechanism of amyloid formation in different proteins involved in neurodegenerative diseases such as Parkinson’s and Alzheimer’s diseases (Jain et al; Biophys J 2011 and Biophys J 2016).
Following her PhD, Dr Jain joined the University of Michigan, Ann Arbor, as a postdoctoral research fellow. Her work was focused on understanding the molecular basis of curli (bacterial functional amyloid) inhibition. She implemented small molecules and chaperone-like proteins to reduce biofilm burden. In an effort to develop new strategies to combat antibiotic resistance, she discovered that a human protein, transthyretin, can inhibit bacterial amyloid (curli) formation in vitro and biofilm-formation in vivo (Jain et al, Proc Natl Acad Sci USA 2017).
At Ahmedabad University, Dr Jain intends to develop her studies to understand the effects of bacterial amyloids on human diseases. Her laboratory uses a wide range of microbiological, biophysical and biochemical techniques to answer the questions.
Research at the Jain lab is primarily curosity and question driven. At present, we are focusing on following areas, however we welcome new ideas and collaborations to expand our research:
1. Development of strategies to combat amyloid-dependent biofilm formation
2. Role of microbial amyloids in non-microbial diseases
3. Cross-talk between amyloids of different origin and its consequences
Research Support: The lab is financially supported by the start-up grant (2018-2021) from Ahmedabad University and Early Career Research Award (ECRA) from SERB (2019-2022).
Articles in peer reviewed journals:
- Klein RD, Shu Q, Cusumano Z, Nagamatsu K, Gualberto N, Lynch A, Wu C, Wang W, Wagner N, Jain N, Pinkner J, Hultgren S, Frieden C, and Chapman M. Structure-Function Analysis of CsgE Reveals Regions Essential for Amyloid Fiber Formation in E. coli. Just accepted in mBio.
- Bhasne K, Sebastian S, Jain N, and Mukhopadhyay S. Synergistic Amyloid Switch Triggered by Early Heterotypic Oligomerization of Intrinsically Disordered α-Synuclein and Tau. J. Mol. Biol. (2018), https://doi.org/10.1016/j.jmb.2018.04.020. Selected and evaluated by Faculty of 1000
- Jain N, Ådén J, Nagamatsu K, Evans ML, Li X, McMichael B, Ivanova MI, Almqvist F, Buxbaum JN, and Chapman MR (2017). Inhibition of Curli Assembly and E. coli Biofilm Formation by the Human Systemic Amyloid Precursor Transthyretin. Proc. Natl. Acad. Sci. U.S.A. 2017 Nov 14;114(46):12184-12189.. http://ns.umich.edu/new/releases/25207-researchers-find-protein-that-could-help-fight-antibiotic-resistance
- Arya S, Dogra P, Jain N and Mukhopadhyay S. Detergent-induced Aggregation of an Amyloidogenic Intrinsically Disordered Protein. J. Chem. Sci. (2017) 129: 1817.
- Jain N, Narang D, Bhasne K, Dalal V, Arya S, Bhattacharya M, and Mukhopadhyay S (2016). Direct observation of the intrinsic backbone torsional mobility of disordered proteins. Biophys. J.111:768–774. Selected and evaluated by Faculty of 1000
- Horowitz S, Koepnick B, Martin R, Tymieniecki A, Winburn A, Cooper S, Flatten J, Rogawski D, Koropatkin N, Hailu T, Jain N, Chapman MR, Sikkema A, Skiba M, Maloney F, Beinlich F, Players F, Students UM, Popovi? Z , Baker D, Khatib F, and Bardwell JCA (2016). Determining crystal structures through crowdsourcing and coursework. Nat. Commun. 7:12549.
- Taylor JD, Hawthorne WJ, Lo J, Dear A, Jain N, Meisl G, Andreasen M, Fletcher C, Koch M, Darvill N, Scull N, Escalera-Maurer A, Sefer L, Wenman R, Lambert S, Jean J, Xu Y, Turner B, Kazarian SG, Chapman MR, Bubeck D, de Simone A, Knowles TP, Matthews SJ (2016). Electrostatically-guided inhibition of curli amyloid nucleation by the CsgC-like family of chaperones. Sci. Rep. 6:24656.
- Chorell E, Andersson E, Evans ML, Jain N, Götheson A, Åden J, Chapman MR, Almqvist F, and Wittung-Stafshede P (2015). Bacterial chaperones CsgE and CsgC differentially modulate human α-synuclein amyloid formation via transient contacts. PLoS ONE 10(10): e0140194.
- Jain N, Bhasne K, Hemaswasthi M, and Mukhopadhyay S (2013). Structural and dynamical insights into the membrane-bound α-Synuclein. PLoS ONE 8(12):e83752.
- Bhattacharya M*, Jain N*, P. Dogra, S. Samai and S. Mukhopadhyay (2013). Nanoscopic amyloid pores formed via stepwise protein assembly. J. Phys. Chem. Lett. 4:480-485. *Joint first author.
- Jain N, Bhattacharya M, and Mukhopadhyay S (2011). Chain collapse of an amyloidogenic intrinsically disordered protein. Biophys. J. 101:1720-1729. Selected and evaluated by Faculty of 1000
- Bhattacharya M, Jain N, and Mukhopadhyay S (2011). Insights into the mechanism of aggregation and fibril formation from bovine serum albumin. J. Phys. Chem. B 115:4195–4205.
- Bhattacharya M, Jain N, Bhasne K, Kumari V and Mukhopadhyay S (2011). pH-induced conformational isomerization of bovine serum albumin studied by extrinsic and intrinsic protein fluorescence. J. Fluoresc. 21:1083-1090.
- Jain N, Bhattacharya M and Mukhopadhyay S (2011). Kinetics of surfactant-induced aggregation of lysozyme studied by fluorescence spectroscopy. J. Fluoresc. 21:615-625.
- Kumar S, Jain N and Mukherjee TK (2009). Role of RAGE in idiopathic pulmonary ?brosis:Promising prospects. Resp. Physiol. Neurobiol. 165:121–122.
- Shivaprakash MR, Jain N, Gupta S, Baghela A, Gupta A and Chakrabarti A (2009). Allergic fungal rhinosinusitis caused by Neosartorya hiratsukae from India. Medical Mycology 1-4.
- Handbook of Infection and Alzheimer’s disease J. Miklossy (Ed.) IOS Press (5) 297-311
- Jain N and Mukhopadhay S (2015). Applications of fluorescence anisotropy in understanding protein conformational disorder and aggregation. Applied Spectroscopy and the Science of Nanomaterials. Progress in Optical Science and Photonics. 8:41-57.
Currently, I am offering following courses:
1. Ethics in Life Sciences: Winter semester
2. Biophysics: Winter semester
3. Basic Immunology: Monsoon semester
PhD Programme Chair: Biological and Life Sciences, School of Arts and Sciences, Ahmedabad University
Member: Gradute faculty committee, Ahmedabad University
American Society for Microbiology (ASM)
Neha Jain (Principla Investigator)
Abdulkhalik Mansuri: PhD student rotating for WInter 2019 semester. Abdul is looking at region specific interactions between amyloidogenic proteins of different origin.
Ms. Azba Shaikh: Summer intern (May-July 2018): Azba is pursuing MS in Cognitive neuroscience from Indian Institute of Technology, Gandhinagar (IITGN). Her internship work focuses on identification of amyloidogenic proteins from gut microbes.
Join the lab:
If your research interests broadly matches with ours, please write to me. Currently, I am accepting applications from:
Ph.D. candidates : admission through Ahmedabad University. Please check the link https://ahduni.edu.in/dbls/admissions/ph-d-in-life-sciences
Postdoctoral Fellows: I do not have funds to support postdoctoral fellows as of now. However, if you are really interested, I can help in finding an extramural funding to support your proposal.
MS thesis project: Interested candidates may write driectly to me for one year MS thesis project.
Centre of Excellence, Division of Biological & Life Sciences,