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Research Facilities


Scanning Electron Microscope (SEM)

The scanning electron microscope (SEM) uses a focused beam of high-energy electrons to generate a variety of signals at the surface of specimens. SEMs reveal information about the sample including external morphology (texture), chemical composition, and crystalline structure and orientation of materials making up the sample. Surface structures are observed by secondary electrons, distribution of materials in a specimen is observed by backscattered electrons, and elements are analyzed by EDS.

Specifications  
Model Jeol JSM 6010 LA
Resolution & Magnification 4nm-15nm & 3, 00,000
Voltage 0.5 to 20kV
Filament Tungsten hairpin filament
EDS system  
Download Requisition Form  

Transmission Electron Microscope (TEM)

A Transmission Electron Microscope (TEM) utilizes energetic electrons to provide morphologic, compositional and crystallographic information on samples. At a maximum potential magnification of 1 nanometer, TEMs are the most powerful microscopes. TEMs produce high-resolution, two-dimensional images, allowing for a wide range of educational, science and industry applications.

Specifications  
Model Jeol JEM1400 Plus
Resolution & Magnification 0.2nm & 6, 00,000
Voltage 20 to 120kV
Filament Tungsten hairpin filament/LaB6 Filament
Download Requisition Form  

Ultramicrotome

Ultramicrotomy is a method for cutting specimens into extremely thin slices, called ultra-thin sections, that can be studied and documented at different magnifications in a transmission electron microscope (TEM).[1] It is used mostly for biological specimens, but sections of plastics and soft metals can also be prepared. Sections must be very thin because the 50 to 125 kV electrons of the standard electron microscope cannot pass through biological material much thicker than 150 nm. For best resolutions, sections should be from 30 to 60 nm.

Specifications  
Model Leica UC7
Magnification 9.6x-77x
Metal vacuum chamber
Height adjustable specimen table (Ø 80 mm)to hold 18 SEM Stubs

Flow Cytometer

Flow cytometry is a technology that simultaneously measures and then analyzes multiple physical characteristics of single particles, usually cells, as they flow in a fluid stream through a beam of light. The properties measured include a particle’s relative size, relative granularity or internal complexity, and relative fluorescence intensity. These characteristics are determined using an optical-to-electronic coupling system that records how the cell or particle scatters incident laser light and emits fluorescence.

Specifications  
Model BDCalibur
Laser Air-cooled argon-ion laser, 15 milliwatt, 488 nm
Fluorescence detectors and filters Up to four high-performance, high dynamic range photomultipliers with bandpass filters: 530 nm (FITC), 585 nm (PE/PI), and >670 nm (PerCP) with base unit, and optional 661 nm (APC) with FL4 option
Software BD Cellquest Pro
Download Requisition Form

Usage Rates:

For Acquiring samples: 500/- per sample

Analysis can be done for six(6) samples only


Fluorescent Microscope

The technique of fluorescence microscopy has become an essential tool in biology and the biomedical sciences, as well as in materials science. The application of an array of fluorochromes has made it possible to identify cells and sub-microscopic cellular components with a high degree of specificity amid non-fluorescing material. Fluorescence microscope is capable of revealing the presence of a single molecule. Through the use of multiple fluorescence labeling, different probes can simultaneously identify several target molecules simultaneously.

Specifications  
Model Leica DM 2500
Light Source Halogen
Lens System 10x, 40x, 60x, 100x
Ultraviolet excitation dyes such as DAPI and Hoechst
Blue excitation FITC and related dyes
Green excitation TRITC, Texas Red, etc
Moreover DIC images can be captured  
Download Requisition Form Usage Rates: 2000/- per hour

Dynamic Light Scattering

Dynamic light scattering (DLS), sometimes referred to as Quasi-Elastic Light Scattering (QELS), is a non-invasive, well-established technique for measuring the size and size distribution of molecules and particles typically in the submicron region, and with the latest technology lower than 1nm.

Specifications  
Parameters measured Size, Zeta potential, Molecular weight, A2
Temperature control range 0ºC to 90ºC +/- 0.1ºC
Condensation control Purge using dry air
Standard laser 4mW, 633nm
Correlator 25ns to 8000s, max 4000 channels
Download Requisition Form

400/- per sample for Sample size or Zeta potential

600/- per sample for both sample size and zeta potential


Fourier transform infrared spectroscopy (FTIR)

Fourier transform infrared spectroscopy (FTIR) is a technique which is used to obtain an infrared spectrum of absorption or emission of a solid, liquid or gas. FTIR spectroscopy is based on the vibrational excitation of molecular bonds by absorption of infrared light energy (only the middle infrared section). The sum of vibrational spectra for a cell macromolecule (nucleic acids, proteins, lipids, polysaccharides, etc.) can produce an infrared absorption spectrum that looks like a molecular “fingerprint” for such biological material.

Specifications  
Model Perkin Elmer Spectrum-two
Wavelength Range 8,300 – 350 cm-1
Interferometer Rotary Michelson interferometer
Optical Windows KBr (standard)
Download Requisition Form

Usage Rates:400/- per sample

(Both liquid and solid sample can be processed)


BOD Incubator

BOD incubators are testing equipment that is widely used in microbiological laboratories to maintain and cultivate the cell cultures and microbiological cultures. The instruments are used to sustain and control the humidity and temperature conditions inside the laboratories. The device is also used to maintain certain factors of the atmosphere as carbon dioxide content, oxygen content. Incubators are essential to perform many types of experiments in molecular biology, microbiology and biology cells to culture the eukaryotic cell and bacterial cells. Panasonic Cooled Incubators incorporate a high precision microprocessor temperature control combined with a heater PID and compressor ON-OFF system.

Specifications  
Model Panasonic MIR-154
Volume 123 Ltrs
Temp range 10OC to +60OC

Sputter Coater

Coating of samples is required in the field of electron microscopy to enable or improve the imaging of samples. Creating a conductive layer of metal on the sample inhibits charging, reduces thermal damage and improves the secondary electron signal required for topographic examination in the SEM. Fine carbon layers, being transparent to the electron beam but conductive, are needed for x-ray microanalysis, to support films on grids and back up replicas to be imaged in the TEM. The coating technique used depends on the resolution and application. Sputter coating in scanning electron microscopy is a sputter deposition process to cover a specimen with a thin layer of conducting material, typically a metal, such as a gold/palladium (Au/Pd) alloy. A conductive coating is needed to prevent charging of a specimen with an electron beam in conventional SEM mode (high vacuum, high voltage). While metal coatings are also useful for increasing signal to noise ratio.

Specifications  
Model Leica EM ACE200
Gas Supply Argon gas

Fluorescence Spectrophotometer

Fluorescence spectrophotometry is a class of techniques that assay the state of a biologicalsystem by studying its interactions with fluorescent probe molecules. This interaction ismonitored by measuring the changes in the fluorescent probe optical properties. The Cary Eclipse Spectrophotometer uses a Xenon flash lamp for superior sensitivity, high signal-to-noise, and fast kinetics. It measures the emission of light from samples in four modes. Using Xenon lamp technology, it captures a data point every 12.5 ms and scans at 24,000 nm/min without peak shifts. The Cary Eclipse is the only spectrophotometer with room light immunity.

Specifications  
Model Agilent Cary Eclipse
Sensitivity >500:1 RMS, 500 nm excitation, excitation and emission slits 10 nm / > 750:1 RMS, 350 nm excitation, excitation and emission slits 10 nm
Temperature Range 10 – 35°C
Wavelength(s) 190 - 1100 nm
Software Cary Eclipse Software
Optical System Cerny-Turner 0.125 m Monochromators
Light Source Xenon Flash Lamp

ELISA Multiplate Reader:

Specifications  
Model BioTek, Synergy HT
Temperature Range 18 – 40°C
Optics λ range: 200 to 999 nm, λ accuracy: ± 2 nm, λ repeatability: ± 0.2 nm, λ bandpass: 2.4 nm
Software Biotek Gen5
Light Source Xenon flash light source
Fluorescence

Tungsten quartz halogen

Excitation Filter: 485/20 nm & Emission Filter: 528/20 nm

Absorbance Measurement Range: 0.000 to 4.000 OD
Download Requisition Form

300/- sample/plate

Extra Charges for Fluorescent Plate / Black Bottom Plate Usage : Rs. 500/-

 ThermoCycler:

Specifications  
Model Eppendorf
Thermo Blocks 96 well plate and 25 well plate, 0.2 & 0.5mL Tubes
Download Requisition Form

PCR: 500/- per complete cycle.

Gradient PCR: 800/- per complete cycle

 

Cell Culture Facility:

The laboratories at the institute are fully equipped with facilities for mammalian cell culture, microbial cell culture. Equipment such as Biosafety Cabinets-level II, DNA/RNA UV cleaner, Laminar Air Flow Hoods, Thermocyclers, Refrigerated Centrifuges, Spectrophotometers, ELISA Plate Reader, BOD Incubators, Eporator and other basic instrumentation needed to conduct research work and practicals are also available at the Institute.

Storage Facility:

In order to store cultures, chemicals and media, storage facility at 4° C, -20° C and -80° C are available at the Institute.

Incubators:

Shaking incubators for aeration and agitation of various cultures and CO2 incubators with humidified environment for the growth of primary cultures and cell lines are also available at the Institute.

 

Note:

    ** For the usage charges of Transmission and Scanning Electron Microscope please contact faculty in-charge Dr. Ritesh K Shukla or email: ritesh.shukla@ahduni.edu.in

     Usage charges are payable in advance by demand draft in favour of “Ahmedabad University”, payable at Ahmedabad. Please note the following:

    •  Service tax extra 18% as applicable as per government norms for all the instrument usages.
    • Requisition form for the use of facility and payment should be sent preferably in the same cover at least one week before the analysis. Samples will be not analysed till payment is received.
    •  A minimum of 5 samples per lot or one hour for any type of analysis are accepted at a time.
    •  The analytical data/spectra are provided only for research/development purposes. These cannot be used as certificates in legal disputes.
    •  Unstable and explosive compounds are not accepted for analysis.
    •  Services are rendered to only those users who regularly give us feedback about the end-use of the results, e.g., thesis, patent, process, publication etc.
    •  All concerned persons, research fellows and students are advised to send their application and samples through their supervisors or Head of Department. The request should be made only on University/College/Institute letter head.
    •  Data analysis and its interpretation are not done normally. However, in special cases this service can be provided on payment basis.